PROCEDURE FOR PERFORMING BUBBLE POINT TEST.

  Procedure for performing Bubble point test.

o   Requirements:

§  Bubble point test assembly.

o   Procedures:

§  Remove the sterilized membrane filter assembly in sterile area.

§  Attach sterile silicon tubing at both ends.

§  Fill 2 liters sterile water for injection in bubble point test apparatus.

                              BUBBLE POINT TESTER

§  Attach upper end of silicon tube to the bubble point test apparatus and lower end to another sterile glass flask.

§  Filter 2 liters of sterile distilled water through membrane using Nitrogen pressure.

§  Remove the upper silicon tube and attach Nitrogen tube to the upper end of membrane holder.

§  Gradually increase the Nitrogen pressure up to 25 PSI  (1.5 lbs) and check for 30 seconds.

§  No bubble should come out from lower end of lower tube (which is dipped in the sterile distilled water) and there should be no fall in pressure,

                                Forms and Records

o   Nil