The procedure for performing Bubble point test.

 Procedure for performing Bubble point test.

Procedure

Precautions And Instructions:

Bubble point testing of membrane filter assembly should be performed before and after aseptic filtration of the solution by manufacturing chemist, and record there of should be maintained in the B.M.R.

Requirements:

                              Bubble point test assembly.

Procedures:

Remove the sterilized membrane filter assembly in sterile area.

Attach sterile silicon tubing at both ends.

Fill 2 liters sterile water for injection in bubble point test apparatus.

Attach upper end of silicon tube to the bubble point test apparatus and lower end to another sterile glass flask.

Filter 2 liters of sterile distilled water through membrane using Nitrogen pressure.

Remove the upper silicon tube and attach Nitrogen tube to the upper end of membrane holder.

Gradually increase the Nitrogen pressure up to 25 PSI  (1.5 lbs) and check for 30 seconds.

No bubble should come out from lower end of lower tube (which is dipped in the sterile distilled water) and there should be no fall in pressure,